Atellica® Solution Hemoglobin A1c Enzymatic Assay Video

Meet Chang, a new technician at a diagnostic testing laboratory. Chang notices there is a higher demand for hemoglobin A1C, or H BA1C tests. He understands HBA 1C level reflects the mean glucose concentration over the past 8 to 12 weeks because HB A1C level is a better indicator of long term glycemic control. And blood and urinary glucose determinations. Physicians use it to diagnose and monitor patients with diabetes myelitis, but also to identify patients at risk for developing this disease. There are various methodologies used to measure HBA 1C levels, such as high performance liquid chromatography or HPLC immunoassay or enzymatic. Curious which methodology is used in his facility and why? Chang asked his supervisor, doctor Morris. Doctor Morris says their facility uses the Siemens healthineers enzymatic hemoglobin A1C assay on the Italica ACH analyzer. Based on a colorimetric enzymatic method, it's a cost effective and efficient solution to consolidate all our testing on one system and meet our increasing testing demands without compromising the assay quality. This fully automated assay requires no manual intervention, which improves the laboratory efficiency and workflow. The whole blood samples are loaded directly on the instrument. The patients HBA 1C and total hemoglobin are automatically measured and their ratio calculated. Cheng wonders how. The whole blood sample is automatically pretreated with a humanizing agent to lies the red blood cells and release the hemoglobin. The hemoglobin is oxidized to produce met hemoglobin. The first reagent is added in the first step of the reaction of fructose al dipeptide fragment is cleave from the hemoglobin beta chain of HBA 1C. Concurrently, met hemoglobin is converted into a more stable form and the total hemoglobin is measured. Next, the second reagent is added. This contains the enzyme protozoal peptide oxidase which breaks down the fructose AL dipeptides and releases. Hydrogen peroxide in the presence of peroxidase hydrogen peroxide produces oxygen which reacts with the coloring agent. The color change is measured to determine the amount of H BA1C in the sample. Cheng asks how the results are reported. Doctor Morris says National Glycohemoglobin standardization program, or NSP, results can be directly related to clinical outcomes and treatment goals, whereas IFC results are accuracy based. Depending on the laboratory, results may be reported in Ifcc or NGDP units using the HBA 1C and total hemoglobin values. The HBA 1C ratio is calculated either in millimoles per mole for IFC units or percent HBA 1C for NGS P HB. A1C results help classify a patient as diabetic or at risk for developing diabetes myelitis. In addition to monitoring the long-term glucose control in patients diagnosed with diabetes. The enzymatic HBA 1C assay offers confidence in results and comes with both NGC certification and IFCC standardization. Its accuracy is demonstrated through the correlation to the reference method. Moreover, it has accuracy with precision performance of less than 2% and is aligned with CAP grading criteria requirements of less than 5% bias. What about analytical interference? By hemoglobin variants? Cheng asks. Dr Morris explains hemoglobin variants may interfere with some assays like immunoassay. This is because changes to the end terminal amino acids can affect the antibodies binding to this region. Since the enzymatic HBA 1C assay can still react with the end terminal of the variance, it reduces analytical interferences from hemoglobin variants. In fact, the assay demonstrates less than five. Percent interference for most common hemoglobin variants. Shane thanks Doctor Morris. Fascinated by what he's learned, he looks forward to providing HBA 1C test results for patients.

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